Simultaneous Analyses of Neutral Carbohydrates and Amino Sugars in Freshwaters with HPLC-PAD
نویسندگان
چکیده
منابع مشابه
Simultaneous analyses of neutral carbohydrates and amino sugars in freshwaters with HPLC-PAD.
In this study, we determine concentrations of neutral and amino sugars and a sugar alcohol in freshwaters using high-performance liquid chromatography and pulsed amperometric detection with a single isocratic analysis. Coeluting arabinose, galactosamine, and mannosamine are separated with a mobile phase of 22.8 mM NaOH-KOH at a temperature of 17 degrees C. The resolutions are 0.73 and 0.64, res...
متن کاملA novel ion-exclusion chromatography-mass spectrometry method to measure concentrations and cycling rates of carbohydrates and amino sugars in freshwaters.
The concentrations of free neutral carbohydrates and amino sugars were determined in freshwater samples of distinct matrix complexity, including meso-, eu- and dystrophic lakes and ponds, using high-performance ion-exclusion chromatography (HPIEC) coupled to mass spectrometry (MS). In contrast to other methods, our approach allowed the quantification of free neutral carbohydrates and amino suga...
متن کاملA novel ion - exclusion chromatography - mass spectrometry method to measure 1 concentrations and cycling rates of carbohydrates and amino sugars in freshwaters 2 3
The concentrations of free neutral carbohydrates and amino sugars were determined in freshwater samples of distinct matrix complexity, including meso-, euand dystrophic lakes and ponds, using high-performance ion-exclusion chromatography (HPIEC) coupled to mass spectrometry (MS). In contrast to other methods, our approach allowed the quantification of free neutral carbohydrates and amino sugars...
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The primary role of carbohydrates (sugars and starches) is to provide energy to cells in the body, particularly the brain, which is the only carbohydrate-dependent organ in the body. The Recommended Dietary Allowance (RDA) for carbohydrate is set at 130 g/d for adults and children based on the average minimum amount of glucose utilized by the brain. This level of intake, however, is typically e...
متن کاملSimultaneous determination of nucleotide sugars with ion-pair reversed-phase HPLC.
Nucleotide sugars are important in determining cell surface glycoprotein glycosylation, which can modulate cellular properties such as growth and arrest. We have developed a conventional HPLC method for simultaneous determination of nucleotide sugars. A mixture of nucleotide sugars (CMP-NeuAc, UDP-Gal, UDP-Glc, UDP-GalNAc, UDP-GlcNAc, GDP-Man, GDP-Fuc and UDP-GlcUA) and relevant nucleotides wer...
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ژورنال
عنوان ژورنال: Journal of Chromatographic Science
سال: 2003
ISSN: 0021-9665,1945-239X
DOI: 10.1093/chromsci/41.8.434